Immunogenicity
HLA-binding assays
Automated, reproducible, highly sensitive, based on a unique collection of HLA class II molecules
Description
A protein can trigger an immune reaction if at least a portion of its sequence, described as epitopes, or peptides, can bind to an HLA class II molecule, and if helper T-cells can be activated by this complex.
The binding of a peptide with HLA class II molecule is thus a necessary, although not sufficient, condition for the immunogenicity. As HLA is highly polymorphic, this accounts for inter-individual differences in the susceptibility to develop an immune reaction to a given antigen.
Testing the ability of a peptide to bind to an HLA class II molecule provides an useful clue on its capacity to induce a potential immune response in individuals possessing this particular molecule.
Indicia benefits from a unique collection of HLA class II proteins. The most frequent alleles have been gathered by the CEA, achieving a broad coverage of the human diversity. The Caucasian type population (Europe and USA) is particularly well represented with a 90% coverage.
Completely automated immuno-enzymatic assays are used for testing the affinity of peptides stemming from the fragmentation of the investigated protein for the HLA class II molecules. This technology enables to characterise the specific interaction between the helper T-cell epitopes and the HLA Class II molecules and to identify the HLA molecules that contribute to the protein-specific immune response.
Goals
• Identify and characterise CD4+ epitopes
• Design non immunogenic variants
• Evaluate the immunogenicity potential of short peptides
• Evaluate the frequency of immune response to a molecule in the population
Features
HLA binding assays relies on a unique collection of HLA class II molecules, the most frequent class in the Caucasian population (Europe and USA). Roughly, the collection achieves a 90% coverage of the Caucasian population.
The technique is based on competitive ELISA using a labelled reference peptide for each HLA class II molecule. The analysis is fully automated.
Starting material
Indicia will synthesise the peptides to ensure that they can be used in the assay.
Please provide us with the sequence of your molecule.
Deliverables
A report including:
• QC summary
• Raw data
• Relative binding activity
• Classification of all peptide / HLA couples (strong binding, moderate binding, weak binding, no binding)
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